王际睿 JIRUI WANG

王际睿, 博士, 教授
Jirui Wang, Ph.D., Professor
emails：
jirui.wang@gmail.com;   isphsc@sicau.edu.cn.

长江学者、国家973计划项目（青年）首席科学家、四川省学术技术带头人、四川省突出贡献专家。从事作物种子发育-萌发与谷物品质研究：鉴定作物种子发育相关关键基因TaPHS-3D、TaPGS1等，解析作物品质形成与保持的机制，从麦类种质资源中挖掘优异穗发芽抗性基因并用于抗性育种。在Mol. Plant, Plant Biotechnol. J., New Phyto., PNAS, Seed Science Research等杂志发表论文100余篇；获四川省科技进步一等奖2项、四川省青年科技奖；获授权发明专利2件、制定地方标准6项、参与作物新品种选育2个。

Our group is dedicated to exploring the realms of crop seed biology and grain quality. We have assessed the tolerance levels to pre-harvest sprouting (PHS) in wheat germplasms collected from major cultivation areas worldwide. This involved characterizing the seed development and germination-related genes TaPHS-3D and TaPGS1 and developing new breeding materials resistant to PHS for wheat breeding programs. Our findings have been published in prestigious journals such as Molecular Plant, Plant Biotechnology Journal, New Phytologist, Proceedings of the National Academy of Sciences, and Seed Science Research. Reflecting on the impact of my research and contributions to seed science education, I was honored with the title of Professor by the Yangtze River Scholar Program in 2022.

教育经历 Education：

l 2004.9-2008.7

Ph.D. 作物遗传育种（Plant Breeding）, 四川农业大学（Sichuan Agricultural University）

l 2001.9-2004.7

MSc作物遗传育种（Plant Breeding）, 四川农业大学（Sichuan Agricultural University）

l 1997.9-2001.7

BSc 生化与分子生物学（Biochemistry & Molecular biology）, 兰州大学（Lanzhou University）

学术与职业经历 Academic & Professional Experience：

l 四川农业大学小麦研究所（Triticeae Research Institute, Sichuan Agricultural University, Chengdu Sichuan, 2008-Now）

The main works in our group are 1) characterization of genes related to seed development and germination in cereals, 2) PHS-resistant wheat breeding, and 3) wheat quality enhancement.

l 美国加州大学戴维斯（UC DAVIS, USA, Apr 2011-Mar 2013）

I worked with Drs. Mingcheng Luo and Jan Dvorak during 2011-2013 as postdoc fellows. We studied the adaptive evolution of the D genome donor of hexaploid wheat, Ae.tauschii. NSF and USDA supported this project.

l 加拿大农业部渥太华研究中心（ECORC-AAFC, Ottawa, ON, Canada, Jan 2007- Feb 2008）.

A project about characterizing seed genes with specific expression levels in Fusarium-infected wheat was carried out.

l 西南作物基因资源与遗传改良教育部重点实验室主任（Director of the Ministry of Education Key Laboratory for Crop Genetic Resources and Improvement in Southwest China, 2020-now）

l 食品安全检测（四川省）重点实验室学术委员会委员（2017-now）

l 固态酿造技术（四川省）创新中心专家委员会委员（2022-now）

l 国际谷物穗发芽大会主委会委员/主席（Committee member of the 14^th and 16^th International Symposium of Pre-harvest sprouting on Cereals）

l 国际植物休眠会议组委会委员（Committee member of the 7^th International Plant Dormancy Symposium）

l <种子科学研究>特邀编辑（Guest Editor of <Seed Science Research>, 2019-2021）

l <农学>编辑（Editor of <Agronomy>, 2021-now）

l <种子>编辑（Editor of <Seeds>, 2022-now）

 

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授课课程Courses

l 种子生物学Seed Biology, 种子科学与技术本科生课程 春季学期

l 种子科学研究进展Advance in Seed Science, 作物遗传育种博士生课程 秋季学期

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研究及招生方向 Current Research：

l 种子发育与萌发的生物学调控 Seed Development and Germination

结合多组学数据分析与分子生物学技术，构建外源环境因子耦合与内源激素协同调控种子发与萌发的网络，对作物种子发育-萌发关键基因进行鉴定，通过遗传分析及基因编辑等策略明确其功能，达到解析作物种子大小、萌发、寿命等重要性状调控机制的目的。

l 作物品质形成与保持 Crop quality

作物籽粒发育过程中积累的淀粉、蛋白等物质特性决定作物品质；穗发芽(PHS)、陈化等负面影响作物品质。解析品质形成与保持机理，发掘优质性状形成、穗发芽抗性、延缓陈化的关键基因，并解析其内源、外源调控机制，为高产优质作物生物育种奠定基础。

Our group is dedicated to deciphering the complex interactions between environmental factors, epigenetic mechanisms, and gene networks that govern the development, dormancy, and germination of cereal seeds. These elements play crucial roles in several key processes of seed biology, such as embryo development, grain filling, maturation, longevity, and germination. Their influence extends to vital aspects of crop production, including yield, quality, nutrition, and resistance to pre-harvest sprouting (PHS). PHS, characterized by the premature germination of grains on the ear before harvest, leads to a significant reduction in end-use quality through the degradation of starch and protein. Recognized as a global challenge, PHS not only diminishes cereal production but also compromises quality. In response, our recent efforts are centered on identifying PHS-resistant genes and selecting superior breeding materials to mitigate this issue effectively.

部分研究工作 Selected works:

l 节节麦单核苷酸多态性揭示小麦D基因组遗传分化与六倍体小麦地理起源

Aegilops tauschii single nucleotide polymorphisms shed light on the origins of wheat D-genome genetic diversity and pinpoint the geographic origin of hexaploid wheat. New Phytologist, 2013, 198: 925-937

Hexaploid wheat (Triticum aestivum, genomes AABBDD) originated by hybridization of tetraploid Triticum turgidum (genomes AABB) with Aegilops tauschii (genomes DD). Genetic relationships between A. tauschii and the wheat D genome are of central importance for the understanding of the wheat origin and subsequent evolution. Genetic relationships among 477 A. tauschii and wheat accessions were studied with the A. tauschii 10K Infinium single nucleotide polymorphism (SNP) array. Aegilops tauschii consists of two lineages (designated 1 and 2) having little genetic contact. Each lineage consists of two closely related sublineages. A population within lineage 2 in the southwestern and southern Caspian appears to be the main source of the wheat D genome. Lineage 1 contributed as little as 0.8% of the wheat D genome. Triticum aestivum is subdivided into the western and Far Eastern populations. The Far Eastern population conserved the genetic make-up of the nascent T. aestivum more than the western population. In wheat, diversity is high in chromosomes 1D and 2D, and it correlates in all wheat D-genome and A. tauschii chromosomes with recombination rates. Gene flow from A. tauschii was an important source of wheat genetic diversity and shaped its distribution along the D-genome chromosomes.

l 小麦在中国传播历史、适应进化与开花、种子萌发性状选择

Uncovering the dispersion history, adaptive evolution and selection of wheat in China. Plant Biotechnology Journal, 2018, 16: 280-291

Wheat was introduced to China approximately 4500 years ago, where it adapted over a span of time to various environments in agro-ecological growing zones. We investigated 717 Chinese and 14 Iranian/Turkish geographically diverse, locally adapted wheat landraces with 27,933 DArTseq (for 717 landraces) and 312,831 Wheat660K (for a subset of 285 landraces) markers. This study highlights the adaptive evolutionary history of wheat cultivation in China. Environmental stresses and independent selection efforts have resulted in considerable genome-wide divergence at the population level in Chinese wheat landraces. In total, 148 regions of the wheat genome show signs of selection in at least one geographic area. Our data show adaptive events across geographic areas, from the xeric northwest to the mesic south, along and among homoeologous chromosomes, with fewer variations in the D genome than in the A and B genomes. Multiple variations in interdependent functional genes, such as regulatory and metabolic genes controlling germination and flowering time were characterized, showing clear allelic frequency changes corresponding to the dispersion of wheat in China. Population structure and selection data reveal that Chinese wheat spread from the northwestern Caspian Sea region to south China, adapting during its agricultural trajectory to increasingly mesic and warm climatic areas.

l WheatGmap: 小麦基因定位与基因组研究综合平台

WheatGmap: A Comprehensive Platform for Wheat Gene Mapping and Genomic Studies. Molecular Plant, 2021, 14: 187-190

Gene mapping of mutations is a critical step for new gene discovery and functional analysis. However, this step has been limited by the discovery of very dense sets of informative markers. Here, we report a platform, Wheat Genomic Map (WheatGmap, https://www.wheatgmap.org), for the fast and cost-effective mapping of genes in wheat. The main application of this platform is the use of several statistical models enabling researchers to simply and flexibly conduct bulked segregant analysis (BSA)-based causal variation mining and gene mapping. A significant feature of the platform is that large-scale genomic variant data can be used as a resource to filter non-causal SNPs or non-validated candidate genes. Moreover, users can share their genomics data of genetic information and annotated phenotypes via WheatGmap. In the current version (WheatGmap 1.0), more than 3,500 next-generation sequencing datasets, including whole-exome sequencing (WES), whole-genome sequencing (WGS), and RNA-seq datasets from public resources, were processed with our standard pipeline. These online tools and genomics data could become an easily applicable resource for gene mapping in a complex wheat genome background.

l Myb10-D转录因子PHS-3D通过调控ABA合成关键基因NCED增强小麦穗发芽抗性

Myb10-D confers PHS-3D resistance to pre-harvest sprouting by regulating NCED in ABA biosynthesis pathway of wheat. New Phytologist 2021, 230: 1940-1952

Pre-harvest sprouting (PHS), the germination of grain before harvest, is a serious problem resulting in wheat yield and quality losses. Here, we mapped the PHS resistance gene PHS-3D from synthetic hexaploid wheat to a 2.4 Mb presence-absence variation (PAV) region and found that its resistance effect was attributed to the pleiotropic Myb10-D by integrated omics and functional analyses. Three haplotypes were detected in this PAV region among 262 worldwide wheat lines and 16 Aegilops tauschii, and the germination percentages of wheat lines containing Myb10-D was approximately 40% lower than that of the other lines. Transcriptome and metabolome profiling indicated that Myb10-D affected the transcription of genes in both the flavonoid and ABA biosynthesis pathways, which resulted in increases in flavonoids and ABA in transgenic wheat lines. Myb10-D activates NCED by biding the secondary wall MYB-responsive element (SMRE) to promote ABA biosynthesis in early wheat seed development stages. We revealed that the newly discovered function of Myb10-D confers PHS resistance by enhancing ABA biosynthesis to delay germination in wheat. The PAV harboring Myb10-D associated with grain color and PHS will be useful for understanding and selecting white-grained PHS resistant wheat cultivars.

    

l bHLH转录因子TaPGS1调控FL3蛋白影响谷物籽粒生长与产量

The PGS1 basic helix-loop-helix (bHLH) protein regulates Fl3 to impact seed growth and grain yield in cereals. Plant Biotechnology Journal 2022, online

Plant transcription factors, such as basic helix-loop-helix (bHLH) and AT-rich zinc-binding proteins (PLATZ), play critical roles in regulating the expression of developmental genes in cereals. We identified the bHLH protein TaPGS1 (T. aestivum Positive Regulator of Grain Size 1) specifically expressed in the seeds at 5–20 days post-anthesis in wheat. TaPGS1 was ectopically overexpressed (OE) in wheat and rice, leading to increased grain weight (up to 13.81% in wheat and 18.55% in rice lines) and grain size. Carbohydrate and total protein levels also increased. Scanning electron microscopy results indicated that the starch granules in the endosperm of TaPGS1 OE wheat and rice lines were smaller and tightly embedded in a proteinaceous matrix. Furthermore, TaPGS1 was bound directly to the E-box motif at the promoter of the PLATZ transcription factor genes TaFl3 and OsFl3 and positively regulated their expression in wheat and rice. In rice, the OsFl3 CRISPR/Cas9 knockout lines showed reduced average thousand-grain weight, grain width, and grain length in rice. Our results reveal that TaPGS1 functions as a valuable trait-associated gene for improving cereal grain yield.

Lab News:

20240227：郎静等论文“Variation of TaMyb10 and their function on grain color and pre-harvest sprouting resistance of wheat”在The Plant Journal发表（DOI: 10.1111/tpj.16676）。

20231101：王际睿、郎静参加第20届中国作物学会学术年会（长沙, 2023）。王际睿教授做“小麦穗发芽抗性资源发掘与育种利用”大会报告。郎静获得“袁隆平追梦奖学金（博士研究生）”。

20231012：梁王壮等论文“Identification of long-lived and stable mRNAs in the aged seeds of wheat”在Seed Biology在线发表（DOI: 10.48130/SeedBio-2023-0014）。

20231007: 王际睿、郎静参加第十五届国际谷物穗发芽大会（Tsukuba, 2023）。王际睿教授完成了国际谷物穗发芽大会组委会轮值主席任务并继续作为成员参与第16届工作。会议期间讨论并确定增加欧洲地区协作组成员、继续与国际种子科学学会的战略合作、拟与知名杂志社合作出版并发表近期研究进展。

20230917：王际睿、符语昕参加第7届国际植物休眠研讨会（Perth, 2023）。符语昕同学设计了本次国际研讨会的会徽并荣获7th IPD最佳报告奖。

20230609: 唐豪等论文“Identification of candidate gene for the Defective Kernel (Dek) phenotype using bulked segregant RNA and exome capture sequencing methods in wheat”在Frontiers in Plant Science在线发表（DOI: 10.3389/fpls.2023.1173861）

20221106: 郎静、郭晓江、林媛媛在中国作物学会作物种子专业委员会2022年学术年会上做的学术报告分别获得二等奖、三等奖、三等奖。

20220322: 郭晓江等论文“The PGS1 basic helix-loop-helix (bHLH) protein regulates Fl3 to impact seed growth and grain yield in cereals” 在Plant Biotechnology Journal在线发表（DOI: 10.1111/pbi.13809）。

20220224: 何雨等论文“Temporal transcriptomes unravel the effects of heat stress on seed germination during wheat grain filling” 在Journal of Agronomy And Crop Science在线发表（DOI: 10.1111/jac.12586）。

20211208：唐豪等论文“Genome-wide association studies of grain black point resistance in Chinese wheat landraces”在Plant Disease在线发表（DOI: 10.1094/PDIS-09-21-1898-RE）。

20210809-13：参加13届国际种子科学大会ISSS-2020（Online, UK）。

20210326: 肖春生等论文“Characterization and expression quantitative trait loci analysis of TaABI4, a pre-harvest sprouting related gene in wheat” 在Seed Science Research发表（DOI: 10.1017/S0960258521000015）。

20210302: 郎静等论文“Myb10-D confers PHS-3D resistance to pre-harvest sprouting by regulating NCED in ABA biosynthesis pathway of wheat” 在New Phytologist发表（DOI: 10.1111/nph.17312）。

20210203: 张立超、董春豪、陈中旭等论文“WheatGmap: A Comprehensive Platform for Wheat Gene Mapping and Genomic Studies” 在Molecular Plant发表（DOI: 10.1016/j.molp.2020.11.018）。

20201108: 符语昕等论文“Identification and Characterization of PLATZ Transcription Factors in Wheat” 在IJMS发表。

20190829：杨剑等论文“Identification of qPHS.sicau-1B and qPHS.sicau-3D from synthetic wheat for pre-harvest sprouting resistance wheat improvement”在Molecular Breeding发表。

20190730-0802：第十四届“国际谷物穗发芽大会”顺利召开（isphsc.csp.escience.cn）。大会共吸引到了来自中国、美国、日本、澳大利亚、英国等国家80余名行业内专家学者到场，围绕“种子休眠与发芽”、“淀粉酶与穗发芽”、“穗发芽分子遗传机制”、“穗发芽抗性育种”四个专题展开了深入的交流与讨论。刘宇娇、黄雯做会议报告。王际睿当选为第十五届大会（日本筑波，2023）主席。

    感谢课题组及小麦研究所、四川农业大学、四川省农科院等老师、同学们对会议的支持，同时感谢罗氏、博奥晶典、库蓝科技、瀚辰光翼、慧诺瑞德、肖邦技术等公司的协助。

20181117：科技部验收王际睿主持的国家973计划项目“芽麦对品质的影响及抗穗发芽小麦材料全基因组选育”。

20180822：周勇等论文“白皮小麦抗穗发芽资源评价及抗性候选位点关联分析”在麦类作物学报发表。

20180121：周勇等论文“Uncovering the Dispersion History, Adaptive Evolution and Selection of Wheat in China”在Plant Biotechnology Journal发表。

20171214-15：王际睿、程梦萍、陈中旭参加2017年度国家超级计算广州中心“天河二号”超算应用大会，“作物基因组大数据分析”工作获得2017年度优秀应用奖。

20170909-22：王际睿、陈中旭参加12届国际种子科学大会ISSS（Monterey, CA），并做“Characterization of pre-harvest sprouting resistance genes in a large germplasm collection of Chinese wheat landraces & Aegilops tauschii”报告；访问UC Davis。

20170822：郭晓江参加第18届全国植物基因组学大会（兰州），获得优秀墙报奖。

20170715-29：王际睿参加“中-澳青年科学家交流项目”（Australia China Young Scientists Exchange Program）访问澳大利亚CSIRO_Canberra, CSIRO_Brisbane, University of Queensland, Murdoch University, University of West Australia，进行学术交流与报告。

20170702：郭晓江论文“Global identification, structural analysis and expression characterization of bHLH transcription factors in wheat”在BMC Plant Biology发表。

20170610：陈学伟团队完成的“A natural allele of a transcription factor in rice confers broad-spectrum blast"在Cell发表。王际睿、程梦萍、陈中旭进行了该论文大数据分析部分工作。

20170514：王际睿参加第五届全国种子科学与技术学术研讨会（桂林）并做学术报告。

20170429：周勇等论文“Genome-Wide Association Study for Pre-harvest Sprouting Resistance in a Large Germplasm Collection of Chinese Wheat Landraces”在Frontiers in Plant Science发表。

20170228：陈真勇等论文“Identification and positional distribution analysis of transcription factor binding sites for genes from the wheat fl-cDNA sequnces”在Bioscience, Biotechnology, and Biochemistry发表。

20170126：周科等论文“Sequence analysis and expression profiles of TaABI5, a pre-harvest sprouting resistance gene in wheat” 在Gene & Geno发表。

20161227：刘宇娇等论文“Conferring resistance to pre-harvest sprouting in durum wheat by a QTL identified in Triticum spelta”在Euphytica发表。

20160918：王际睿、周勇参加第13届国际谷物穗发芽会议（澳大利亚-佩斯）并分别做主题报告，同时成功申请承办下一届会议。

20160814：王际睿、刘宇娇、谭超参加第7届世界作物学大会（北京），期间王际睿做主题报告。

20160315：陈中旭等论文“SNP mining in functional genes from nonmodel species by next-generation sequencing: ... in wheat”在BMRI发表。

20151112：王际睿、杨剑、周勇参加第7届全国小麦遗传育种研讨会（河南-郑州），周勇获得优秀博士研究生墙报奖。

20150818：王际睿、杨剑、周勇参加第6届中国小麦基因组与分子育种大会（陕西-杨凌），王际睿做大会特邀报告。

20150302：陈真勇、郭晓江等论文“Genome-wide characterization of developmental stage- and tissue-specific transcription factors in wheat”在BMC Genomics发表。

20140915-19：王际睿参加11届国际种子科学大会ISSS（Changsha, China）。

20140408：杨剑等论文“Molecular characterization of high pI α-amylase and its expression QTL analysis in synthetic wheat RILs”被Molecular Breeding接收。

Lab Members:

董慧雪 博士，特聘副教授，硕士生导师

Dong Huixue, PhD, Institute of Crop Science, CAAS

研究方向：作物功能基因组&遗传改良

Current Research: Crop Functional Genomics & Genetics Improvement

陈倩 博士，特聘副教授

Chen Qian, PhD, China Agricultural University

研究及招生方向：小麦品质形成保持机制及优质小麦新材料创制

Current Research:The mechanism of wheat quality formation and preservation & high-quality wheat selection

郭晓江 博士

Guo Xiaojiang, PhD, Sichuan Agricultural University

研究方向：小麦种子发育的遗传调控

Research: Genetic regulation of wheat seed development

程梦萍，讲师

Cheng Mengping, BSc, Chengdu Neusoft University

研究方向：作物生物信息学&数据发掘

Research: Crop Bioinformatics & Data Mining

李茂莲，讲师

Li Maolian, MSc, South West University

研究方向：育种技术-编辑&转化

Research: Breeding technology - Editing & Transformation

Former Research Assistant

王早霞 (Wang Zaoxia), 2013-2016; 龙茜 (Long Xi), 2013-2014; 李净琼 (Li Jingqiang), 2009-2013

Ph.D. Students

陈真勇 (Chen Zhenyong) 2013-2015

杨剑 (Yang Jian) 2012-2016

周勇 (Zhou Yong) 2010-2013-2017

刘宇娇 (Liu Yujiao) 2014-2018; 联合培养: 2014.09-2015.09, AAFC-Ottawa加拿大

陈中旭 (Chen Zhongxu) 2012-2015-2021; 联合培养: 2016.04-2017.09, UC Davis美国

张琴 (Zhang Qin) 2013-2016-2021; 联合培养: 2018.11-2021.2, CSIRO_Canberra澳大利亚

桂李暄 (Gui Lixuan) 2016-2023

郭晓江 (Guo Xiaojiang) 2014-2017-2022; 联合培养: 2018.12-2021.12, UC Davis美国

Iqbal Hussain 2017-2018

唐豪(Tang Hao) 2015-2018-2023

谭超(Tan Chao) 2015-2018-now

郎静 (Lang Jin) 2016-2019-now

符语昕 (Fu Yuxin) 2017-2020-now;联合培养: 2021.4-2023.6, 中国科学院分子植物科学卓越创新中心

何雨 (He Yu) 2020-now

蒲茜 (Pu Xi) 2021-now

Ummar Ali 2021-now

李惠根（Li Huigen）2022-now

梁王壮 (Liang Wangzhuang) 2023-now

Thanapon Prangkratok 2023-now

张钰婷（Zhang Yuting）2023-now

MSc Students

刘昆 (Liu Kun) 2011-2014

朱松 (Zhu Song) 2013-2016

Kwame Obeng Dankwa 2015-2017

肖春生 (Xiao Chunsheng) 2016-2020; 联合培养: 2019.11-2020.04, Murdoch澳大利亚

杨力生 (Yang Lisheng) 2016-2019

黄雯 (Huang Wen) 2017-2020

王祥向 (Wang Xiangxiang) 2017-2020

邓敏 (Deng Min) 2017-2020

张承碧 (Zhang Chengbi) 2018-2021

李令川 (Li Lingchuan) 2019-2022

梁王壮 (Liang Wangzhuang) 2020-2023

李之恒 (Li Zhiheng) 2020-2023

杨珞 (Yang Luo) 2021-now

林媛媛 (Lin Yuanyuan) 2021-now

张文鹏 (Zhang Wenpeng) 2021-now

王哲浩（Wang Zhehao）2022-now

袁艺伟（Yuan Yiwei）2022-now

刘馨（Liu Xin）2022-now

赵庆昊（Zhao Qinhao）2023-now

杨丹宁（Yang Danning）2023-now

俄洛阿依（Eluo Ayi）2023-now

金雨梦（Jin Yumeng）2023-now

Undergraduate Students

2022:古敬(Gu Jin), 刘佳芸 (Liu Jiayun), 曹馨引 (Cao Xinyin), 王铭尉 (Wang Mingwei)，文霖愉 (Wen Linyu)

2021: 唐雅楠 (Tang Yanan), 陈宏宇 (Chen Hongyu), 许力木 (Xu Limu), 刘禄宏 (Liu Luhong), 徐茂祥 (Xu Maoxiang)

2019: 宋秋池 (Song Qiuci)

2017: 吴晓葭 (Wu Xiaojia)2016: 郭世宽 (Guo Shikuan), 曾小玉 (Zeng Xiaoyu), 李晶 (Li Jin), 端木笑盈 (Duanmu Xiaoying), 王冉 (Wang Ran), 张承碧 (Zhang Chenbi), 李承志 (Li Chengzhi)

2015: 马欣源 (Ma Xinyuan)

2014: 符语昕 (Fu Yu-Xin)

2013: 毛瑞文 (Mao Ruiwen), 谢娇 (Xie Jiao)

2012: 余璨 (Yu Can), 陈文帅 (Chen WenShuai), 肖春生 (Xiao Chunsheng), 陈丽娜 (Chen Lina)

2011: 周科 (Zhou Ke), 段杰 (Duan Jie), 郎静 (Lang Jing)