Identification and QTL analysis of stripe rust resistance in the common wheat cultivar Gaoyuan813

https://link.springer.com/article/10.1007/s11032-025-01616-7

Molecular Breeding，Volume 45, article number 89, (2025)

Kanghui Zeng,Yuqin Li,Liuyang Shang,Yanling Hu,Zhenzhen Wei,Qiang Zhou,Lianquan Zhang,Dengcai Liu,Bo Zhang&Lin Huang

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a major threat to global wheat production. Developing resistant wheat varieties is a crucial objective in wheat breeding programs. The Chinese common wheat cultivar Gaoyuan813 (GY813) has exhibited excellent resistance to Pst under field conditions for several years. The objective of this study was to identify quantitative trait loci (QTLs) associated with stripe rust resistance using a recombinant inbred line (RIL) population derived from a cross between Gaoyuan813 and the susceptible variety Fukuho. The RILs were evaluated for stripe rust resistance in four field environments with a mixture of Chinese prevalent Pst races (CYR32, CYR33, CYR34, Zhong4, and HY46) and in a growth chamber with race CYR34 and genotyped using the Wheat55K single nucleotide polymorphism array. Five QTLs for stripe rust resistances were mapped to chromosomes 1BL (2), 2AS (2), and 7DS (1), explaining 4.37%-25.44% of the phenotypic variance. QYrsicau-2AS.1 and QYrsicau-7DS were stably detected across all field environments, whereas QYrsicau-1BL.2 was only detected in the growth chamber test. QYrsicau-1BL.1 and QYrsicau-7DS may correspond to the known resistance genes Yr29 and Yr18, respectively, while QYrsicau-1BL.2 and QYrsicau-2AS.2 are likely novel. Additive effects were observed for the combination of QYrsicau-1BL.1, QYrsicau-2AS.1, and QYrsicau-7DS. KASP markers linked to QYrsicau-1BL.2 (KASP_AX-109878201) and QYrsicau-2AS.1 (KASP_AX-110981112) were developed and validated to facilitate the breeding use of genes for wheat improvement.