https://link.springer.com/article/10.1007/s11032-025-01589-7
Molecular Breeding,Volume 45, article number73, (2025)
Shuai Hou,Hong Zhou,Jinxiong Lv,Peng Chen,Caixia Li,Yu Lin,Yueyue Liu&Yaxi Liu
Abstract
Tiller number is an essential agronomic characteristic that influences barley morphology and yield. A barley low number of tillers mutant CIHO 11,530 exhibits few tillers and in this study, we conducted a genetic analysis of the barleylow number of tillers 2(lnt2) locus. Linkage analysis showed that lnt2 was mapped in an interval of 3.39 cM on chromosome 6HS between the flanking markers SNP1765 and SNP526, explaining 53.06% of the phenotypic variance. The genetic effect of lnt2was further verified in two other genetic backgrounds, explaining variances of 86.43% and 91.01% in tiller numbers between lines carrying thelnt2mutant and wild-type alleles, respectively. Furthermore, we constructed a large F2 population and fine-mappedlnt2. Finally,lnt2was mapped within a 0.19 cM genetic interval delimited by the tightly linked KASP markers KASP6359and KASP365, and the physical interval was located at 40.57–42.35 Mb. In this interval, three genes were highly likelylnt2based on gene annotations, sequence and gene expression analyses. Our research provides valuable information for the map-based cloning of lnt2.