Molecular Plant Pathology，12 June 2023
Yue Li,Rongrong Zhang,Yu Wu,Qin Wu,Qiantao Jiang,Jian Ma,Yazhou Zhang,Pengfei Qi,Guoyue Chen,Yunfeng Jiang,Youliang Zheng,Yuming Wei,Qiang Xu
The dynamic balance and distribution of sphingolipid metabolites modulate the level of programmed cell death and plant defence. However, current knowledge is still limited regarding the molecular mechanism underlying the relationship between sphingolipid metabolism and plant defence. In this study, we identified a wheat RNA-binding protein 1 (TaRBP1) andTaRBP1mRNA accumulation significantly decreased in wheat after infection byPuccinia striiformisf.sp.tritici(Pst). Knockdown ofTaRBP1via virus-induced gene silencing conferred strong resistance to Pst by enhancing host plant reactive oxygen species (ROS) accumulation and cell death, indicating that TaRBP1 may act as a negative regulator in response to Pst. TaRBP1 formed a homopolymer and interacted with TaRBP1 C-terminus in plants. Additionally, TaRBP1 physically interacted with TaGLTP, a sphingosine transfer protein. Knockdown ofTaGLTPenhanced wheat resistance to the virulent Pst CYR31. Sphingolipid metabolites showed a significant accumulation inTaGLTP-silenced wheat andTaRBP1-silenced wheat, respectively. In the presence of the TaRBP1 protein, TaGLTP failed to be degraded in a 26S proteasome-dependent manner in plants. Our results reveal a novel susceptible mechanism by which a plant fine-tunes its defence responses by stabilizing TaGLTP accumulation to suppress ROS and sphingolipid accumulation during Pst infection.