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Exome Sequencing from Bulked Segregant Analysis Identifies a Gene for All-Stage Resistance to Stripe Rust on Chromosome 1AL in Chinese Wheat Landrace ‘Xiaohemai’

作者:   审稿人:刘宇娇     时间: 2022-05-30 点击次数:


https://apsjournals.apsnet.org/doi/10.1094/PDIS-08-21-1618-RE

Exome Sequencing from Bulked Segregant Analysis Identifies a Gene for All-Stage Resistance to Stripe Rust on Chromosome 1AL in Chinese Wheat Landrace ‘Xiaohemai’

    Yunfeng Jiang, Luyao Duan, Fangnian Guan, Fangjie Yao, Li Long, Yuqi Wang, Xuyang Zhao, Hao Li, Wei Li, Qiang Xu, Qiantao Jiang, Jirui Wang, Yuming Wei, Jian Ma, Houyang Kang, Pengfei Qi, Mei Deng, Youliang Zheng, and Guoyue Chen

    Abstract

    Stripe rust caused byPuccinia striiformisf. sp.triticiis one of the most destructive diseases of wheat. Identifying novel resistance genes applicable for developing disease-resistant cultivars is important for the sustainable control of wheat stripe rust. Chinese wheat landrace ‘Xiaohemai’ (‘XHM’) is an elite germplasm line with all-stage resistance (ASR) effective against predominant ChineseP. striiformisf. sp.triticiraces. In this study, we performed a bulked segregant analysis coupled with exome capture sequencing (BSE-seq) to identify a candidate genomic region strongly associated with stripe rust resistance on chromosome 1AL in 173F2:3lines derived from the cross ‘XHM’ × ‘Avocet S’. The gene, designated asYrXH-1AL, was validated by a conventional quantitative trait locus analysis using newly developed Kompetitive allele-specific PCR (KASP) markers, explaining up to 48.50% of the phenotypic variance. By testing a secondary mapping population comprising 144 lines from the same cross at the seedling stage with prevalentP. striiformisf. sp.triticirace CYR34,YrXH-1ALwas identified as a single Mendelian factor in a 1.5-cM interval flanked by KASP markersKP1A_484.33andKP1A_490.09. This region corresponded to a 5.76-Mb genomic interval on ‘Chinese Spring’ chromosome 1AL. Furthermore, two cosegregating KASP markers showed high polymorphisms among 130 Chinese wheat cultivars and could be used for marker-assisted selection. Because no otherYrgenes for ASR that originated from common wheat have been detected on chromosome 1AL,YrXH-1ALis likely a novel gene that can be incorporated into modern breeding materials to develop wheat cultivars with enhanced stripe rust resistance.

     

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