Development and identification of four new synthetic hexaploid wheat lines with solid stems

https://www.nature.com/articles/s41598-022-08866-x#Abs1

SCIENTIFIC REPORTS，12，4898（2022）

Dongyu Liang,Minghu Zhang,Xin Liu,Hui Li,Zhenjiao Jia,Dinghao Wang,Ting Peng,Ming Hao,Dengcai Liu,Bo Jiang,Lin Huang,Shunzong Ning,Zhongwei Yuan,Xuejiao Chen &Lianquan Zhang

Abstract

Stem solidness is an important agronomic trait for increasing the ability of wheat to resist lodging. In this study, four new synthetic hexaploid wheat with solid stems were developed from natural chromosome doubling of F1hybrids between a solid-stemmed durum wheat (Triticum turgidumssp.durum, 2n = 4x = 28, AABB) and fourAegilops tauschii(2n = 2x = 14, DD) accessions. The solid expression of the second internode at the base of the stem was stable for two synthetic hexalpoid wheat Syn-SAU-117 and Syn-SAU-119 grown in both the greenhouse and field. The lodging resistance of four synthetic solid-stem wheats is stronger than that of CS, and Syn-SAU-116 has the strongest lodging resistance, followed by Syn-SAU-119. The paraffin sections of the second internode showed that four synthetic wheat lines had large outer diameters, well-developed mechanical tissues, large number of vascular bundles, and similar anatomical characteristics with solid-stemmed durum wheat. The chromosomal composition of four synthetic hexaploid wheat was identified by FISH (fluorescence in situ hybridization) using Oligo-pSc119.2-1 and Oligo-pTa535-1. At adult stage, all four synthetic hexaploid wheat showed high resistance to mixed physiological races of stripe rust pathogen (CYR31, CYR32, CYR33, CYR34). These synthetic hexaploid wheat lines provide new materials for the improvement of common wheat.