Disomic 1M (1B) Triticum aestivum–Aegilops comosa Substitution Line with Favorable Protein Properties

https://pubs.acs.org/doi/10.1021/acs.jafc.3c01358

Agricultural and Food Chemistry，2023, 71, 19, 7258–7267

Yuanyuan Zuo,Fanli Jing,Lile Ma,Xinjie Yin,Yuan Wang,Kangle Xiang,Gang Liu,Shoufen Dai，Lianquan Zhang,Dengcai Liu, and Zehong Yan

Abstract

Aegilops comosa(2n= 2x= 14, MM) contains many excellent genes/traits for wheat breeding. Wheat–Ae. comosaintrogression lines have potential value in the genetic improvement of wheat quality. A disomic 1M (1B)Triticum aestivum–Ae. comosasubstitution line NAL-35 was identified by fluorescence in situ hybridization and genomic in situ hybridization analysis from a hybridization cross between a disomic 1M (1D) substitution line NB 4-8-5-9 with CS N1BT1D. The observation of pollen mother cells showed that NAL-35 had normal chromosome pairing, suggesting that NAL-35 could be used for the quality test. NAL-35 with alien Mx and My subunits showed positive effects on some protein-related parameters including high protein content and high ratios of high-molecular-weight glutenin subunits (HMW-GSs)/glutenin and HMW-GS/low-molecular-weight glutenin subunits. The changes in gluten composition improved the rheological properties of the dough of NAL-35, resulting in a tighter and more uniform microstructure. NAL-35 is a potential material for wheat quality improvement that transferred quality-related genes fromAe. comosa.