Genome-Wide Association Study of Kernel Black Point Resistance in Chinese Wheat Landraces

https://apsjournals.apsnet.org/doi/10.1094/PDIS-09-21-1898-RE

Hao Tang, Zhi Tan, Xiangxiang Wang, Lisheng Yang, Guoyue Chen, Hua Yu, Zhien Pu, Qiantao Jiang, Maolian Li, Mengping Cheng, Pengfei Qi, Wei Li, Yujiao Liu, and Jirui Wang

Abstract

Black point (BP) disease of wheat has become a noticeable problem in China. The symptoms are spots that are brown to black in color around the wheat kernel embryo or in the endosperm, resulting in a significant reduction of wheat grain quality. Here, we evaluated 272 Chinese wheat landraces for BP reaction and performed a genome-wide association study to identify BP resistance quantitative trait loci (QTLs) in five field environments without artificial inoculation. The BP incidence data showed continuous distributions and had low to moderate correlations between environments (r= 0.094 to 0.314). Among the 272 landraces, 11 had 0.1 to 4.9%, 144 had 5 to 14.9%, 100 had 15 to 29.9%, and 17 had >30% incidence. We found three resistant accessions: WH094 (3.33%), AS661463 (2.67%), and AS661231 (2.67%), which can be used in breeding programs to enhance BP resistance. We identified 11 QTLs, which explained 8.22 to 10.99% phenotypic BP variation, and mapped them to eight wheat chromosomes. Three of the QTLs were novel. The molecular markers for the BP resistance could facilitate molecular breeding for developing BP-resistant cultivars.