Tiller angle (TA) strongly influences plant architecture and grain yield in cereals. However, the genetic basis of TA in wheat is largely unknown. We identified three TA-related quantitative trait loci (QTL). One of them wasQTa.sau-2B-769, a major QTL localized on chromosome arm 2BL.QTa.sau-2B-769was detected in seven environments, explaining 18.1%–51.1% of phenotypic variance. We developed a linked Kompetitive Allele-Specific Polymerase chain reaction (KASP) marker,KASP-AX-108792274, to further validate this locus in three additional populations in multiple environments.QTa.sau-2B-769increased TA by up to 24.9% in these populations. There were significant and positive correlations between TA and flag leaf angle (FLANG). However, TA was not correlated with plant height or anthesis date, suggesting that expression ofQTa.sau-2B-769is independent of vernalization.TraesCS2B01G583800, a gene known to be involved in leaf angle regulation, was identified as the most likely candidate gene forQTa.sau-2B-769. These results enrich our understanding of the mechanisms regulating wheat TA at maturity and may support precise mapping and cloning of gene(s) underlyingQTa.sau-2B-769.