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Characterization of novel LMW glutenin subunit genes at the Glu-M3 locus from Aegilops comosa

点击数:发布时间:2018-10-12 09:39:03 来源:

Lin Huang,Yu He,Yarong Jin,Fang Wang,Jingshu He,Lihua Feng,Dengcai Liu,Bihua Wu


https://link.springer.com/article/10.1007/s13205-018-1407-8?tdsourcetag=s_pctim_aiomsg


Abstract

We report the characterization of seven novel low-molecular-weight glutenin subunit (LMW-GS) genes from Aegilops comosa (2n = 2x = 14, MM). We found that all seven LMW-GS genes possessed the same primary structure shared by other known LMW-GSs. Three genes (comosa-M1, comosa-M2, and comosa-M3) encode LMW-m-type subunits, two (comosa-I1and comosa-I2) encode LWM-i-type subunits, and two (comosa-L1 and comosa-L2) encode LWM-l-type subunits. The comosa-M1 possessed seven cysteine residues, which resulted from a single-nucleotide polymorphism (SNP) of the G-A transition in the fifth position of the N-terminal sequence. Two l-type subunits, comosa-L1 and comosa-L2, contained nine cysteine residues with an extra cysteine residue located in the signal peptide and repetitive domain. In addition, a long insertion of 13 residues (LGQQPQ8/LVQQPQ8) was present in the end of the C-terminal II. Phylogenetic analysis implied that the comosa-L2 may be the intermediate type during the evolution of LMW-l and LMW-i-type genes. Our results demonstrated that the novel LMW-GSs, such as comosa-M1, comosa-L1, and comosa-L2, may have positive effects on dough properties.


Keywords

Aegilops comosa LMW-GS M genome Wheat quality improvement