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Using a wheat-rye amphihaploid population to map a rye gene responsible for dwarfness

点击数:发布时间:2018-10-12 09:35:49 来源:

Sen Yang,Hongjing Zhu,Jianjun Yu,YuYang Zhong,LaiBin Zhao,YunFeng Jiang,Ming Hao,Lianquan Zhang,Shunzong Ning,XueJiao Chen,Dengcai Liu,Zhongwei Yuan


https://link.springer.com/article/10.1007/s10681-018-2247-7?tdsourcetag=s_pctim_aiomsg


Abstract

Gene identification in cross-pollinating plants such as rye can be arduous and time consuming because of the difficulties involved with genetic population construction. Here, we provide an alternative approach for the construction of mapping populations to rapidly map genes in cross-pollinated cereal rye. The aim of the present experiments was to genetically analyze the dwarf stature expressed by a germplasm accession of rye. The dwarf phenotype was reversible when the seedlings were exposed to gibberellic acid; the reductions in plant height occurred via reductions in cell size. A mapping population was constructed by generating a set of wheat-rye amphihaploids bred from a single rye plant heterozygous for the dwarfing gene(s). The dwarfness phenotype was expressed in the amphihaploid background, and segregation in the mapping population was consistent with the presence of a single gene. Using rye SSR markers, the gene responsible was located on chromosome arm 1RL, which is also the location of the known rye dwarfing gene Ddw3. This gene is valuable for dwarf breeding of wheat as well as rye.

Keywords

Plant height, Dwarf gene, Wheat-rye amphihaploid, Gene mapping