Biosystematics studies on Elymus breviaristatus and Elymus sinosubmuticus (Poaceae: Triticeae)
作者： 审稿人：刁圣轩 时间： 2022-04-02 点击次数：次
http://https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8805286/
Lu Tan，Qing-Xiang Huang，Yang Song，Dan-Dan Wu， Yi-Ran Cheng，Chang-Bin Zhang，Li-Na Sha， Xing Fan， Hou-Yang Kang， Yi Wang，Hai-Qin Zhang，Yong-Hong Zhou
Abstract
Background
Elymus breviaristatusandElymus sinosubmuticusare perennial herbs, not only morphologically similar but also sympatric distribution. The genome composition ofE. sinosubmuticushas not been reported, and the relationship betweenE. sinosubmuticusandE. breviaristatusis still controversial. We performed artificial hybridization, genomic in situ hybridization, and phylogenetic analyses to clarify whether the two taxa were the same species.
Results
The high frequency bivalent (with an average of 20.62 bivalents per cell) at metaphase I of pollen mother cells of the artificial hybrids ofE. breviaristatus(StYH) × E. sinosubmuticuswas observed. It illustrated thatE. sinosubmuticuswas closely related toE. breviaristatus. Based on genomic in situ hybridization results, we confirmed thatE. sinosubmuticuswas an allohexaploid, and the genomic constitution was StYH. Phylogenetic analysis results also supported that this species containedSt,Y, and H genomes. In their F1hybrids, pollen activity was 53.90%, and the seed setting rate was 22.46%. Those indicated that the relationship betweenE. sinosubmuticusandE. breviaristatusis intersubspecific rather than interspecific, and it is reasonable to treatedE. sinosubmuticusas the subspecies ofE. breviaristatus.
Conclusions
In all, the genomic constitutions ofE. sinosubmuticusandE. breviaristatuswereStYH, and they are species in the genusCampeiostachys.BecauseE. breviaristatuswas treated asCampeistachys breviaristata,Elymus sinosubmuticusshould be renamedCampeiostachys breviaristata(Keng) Y. H. Zhou, H. Q. Zhang et C. R. Yang subsp.sinosubmuticus(S. L. Chen) Y. H. Zhou, H. Q. Zhang et L. Tan.
Supplementary Information
The online version contains supplementary material available at 10.1186/s12870-022-03441-y.
Keywords:Campeiostachys, Chromosome pairing, Genomic in situ hybridization, Reproduction isolation, Biosystematics
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