Identification and Mapping of QTL for Stripe Rust Resistance in the Chinese Wheat Cultivar Shumai126

https://apsjournals.apsnet.org/doi/10.1094/PDIS-09-21-1946-RE

Yufan Wang, Yanling Hu, Fangyi Gong, Yarong Jin, Yingjie Xia, Yu He, Yun Jiang, Qiang Zhou, Jingshu He, Lihua Feng, Guoyue Chen, Youliang Zheng, Dengcai Liu, Lin Huang, and Bihua Wu

Abstract

Stripe rust, caused byPuccinia striiformisf. sp.tritici, is a damaging disease of wheat globally, and breeding resistant cultivars is the best control strategy. The Chinese winter wheat cultivar Shumai126 (SM126) exhibited strong resistance toP. striiformisf. sp.triticiin the field for more than 10 years. The objective of this study was to identify and map quantitative trait loci (QTL) for resistance to stripe rust in a population of 154 recombinant inbred lines (RILs) derived from a cross between cultivars Taichang29 (TC29) and SM126. The RILs were tested in six field environments with a mixture of the Chinese prevalent races (CYR32, CYR33, CYR34, Zhong4, and HY46) ofP. striiformisf. sp.triticiand in growth chamber with race CYR34 and genotyped using the Wheat55K single nucleotide polymorphism (SNP) array. Six QTL were mapped on chromosomes 1BL, 2AS, 2AL, 6AS, 6BS, and 7BL, respectively. All QTL were contributed by SM126 exceptQYr.sicau-2AL.TheQYr.sicau-1BLandQYr.sicau-2AShad major effects, explaining 27.00 to 39.91% and 11.89 to 17.11% of phenotypic variances, which may correspond to known resistance genesYr29andYr69, respectively. TheQYr.sicau-2AL,QYr.sicau-6AS, andQYr.sicau-6BSwith minor effects are likely novel.QYr.sicau-7BLwas only detected based on growth chamber seedling data. Additive effects were detected for the combination ofQYr.sicau-1BL,QYr.sicau-2AS, andQYr.sicau-2AL. SNP markers linked toQYr.sicau-1BL(AX-111056129andAX-108839316) andQYr.sicau-2AS(AX-111557864andAX-110433540) were converted to breeder-friendly Kompetitive allele-specific PCR (KASP) markers that would facilitate the deployment of stripe rust resistance genes in wheat breeding.