The KL system in wheat permits homoeologous crossing over between closely related chromosomes

https://www.sciencedirect.com/science/article/pii/S2214514123000053

The Crop Journal, 2023, /doi: 10.1016/j.cj.2023.01.003

Chaolan Fan, Jiangtao Luo, Jiaojiao Sun, Hong Chen, Liqiong Li, Lanyue Zhang, Xue Chen, Yazhou Li, Shunzong Ning, Zhongwei Yuan, Bo Jiang, Lianquan Zhang, Xuejiao Chen, Adam J Lukaszewski, Dengcai Liu, Ming Hao

Abstract

The Chinese wheat landrace Kaixian-Luohanmai (KL) expresses the ph-like phenotype. A major QTL, QPh.sicau-3A (syn. phKL), responsible for this effect has been mapped to chromosome arm 3AL. This study presents some characteristics of homoeologous pairing and recombination induced by phKL. In KL haploids, the level of homoeologous pairing was elevated relative to Ph1 Chinese Spring (CS) haploids. There was a clear preference for A-D pairing and less frequent for A-B and B-D, reflecting the higher levels of affinity between genomes A and D in wheat. The characteristics of pairing were affected by temperature and magnesium ion supplementation. The suitability of phKL for chromosome engineering was tested on three pairs of homoeologues: 2S^v-2B, 2S^v-2D, and 2RL-2BL. The recombination rates were 1.68%, 0.17%, and 0%, respectively. The phKL locus in KL induced a moderate level of homoeologous chromosome pairing and recombination when the Ph1 locus of wheat was present, both in wheat haploids and hexaploids. The Ph1-imposed criteria for chromosome pairing and crossing over were relaxed to some degree, permitting homoeologous crossing over but only between closely related chromosomes; there was no crossing over between more differentiated chromosomes. Therefore, the phKL system (QPh.sicau-3A) can be a useful tool in chromosome engineering of wheat to transfer genes from closely related species with the benefit of reduced genomic chaos generated by the ph1b mutation.